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Peripheral blood mononuclear cells (PBMCs) were collected from the buffy coat, further cleared of the red blood cells, and stored pelleted at 80C until use

Therapeutic doses of vitamin D can differ based on the same variables, but 50,000 IU (1,250 mcg) is a common amount according to the journal Nutrients

While Lumigan is highly effective in reducing eye pressure , one of its most notable side effects is a potential change in eye color
However, the study suggests that further randomized controlled trials (RCTs) are needed in the future to obtain more conclusive results [30]
