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After extensive washing with PBS and then NH 4 CO 3, bound proteins were eluted with 100 mM DTT, concentrated 5-fold and applied to 12% SDS-PAGE gels
However, studies have proposed several mechanisms, including oxidative stress due to elevated reactive oxygen species, mitochondrial dysfunction, release of vasoactive amines, and increased lipid peroxidation 12
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2017;21:472488.e410
