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Description
Progressive increases in ALT activity or those accompanied by evidence of hepatic dysfunction (hyperbilirubinemia, increased serum bile acids, coagulopathy, hypoglycemia, hyperammonemia, hypoalbuminemia) are more likely to represent serious hepatic injury

& Schittenhelm, R

And Night

30 s product amplification at 60 C] and the reaction was set up according to the manufacturers protocol of the QuantiNova SYBR Green PCR Kit (# 208252, Qiagen)
