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Cell suspensions were passed through a 70 m and 40 m cell strainer (BD Bioscience), resuspended in 2.5 mL of 40% Percoll preparation (20 mL Percoll [GE Healthcare], 2.2 mL 10PBS, 27.8 mL HBSS without Ca 2+ and Mg 2+ [Sigma-Aldrich]) and layered onto 1.5 mL 60% Percoll (30 mL Percoll, 3.3 mL 10PBS, 16.7 mL HBSS)
tris(2-carboxyethyl)phosphine) or metal chelators (e.g
17 Their findings supported the use of oral naltrexone and acamprosate as first-line pharmacotherapies for AUD
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Alexander R, Debiec N, Razzaque MS, He P (2022) Inorganic phosphate-induced cytotoxicity
