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Previous data showed that rats subjected to chronic application of WAS for 10 days had visceral hyperalgesia which persisted for at least a month, increased rate of fecal pellet excretion, and evidence of low-grade immune activation such as expression of IL-1 and IFN- stress ( In vitro Models for IBS Human Gut-on-a-Chip The innovation and development of microfluidic organ-on-a-chip models of human intestine have revolutionized the way in which we can study intestinal physiology and pathophysiology

Model induction for DHEA: Mice in the Model group were subjected to daily subcutaneous injections of DHEA (60 mg/kg body weight), dissolved in sesame oil, for 4 weeks to create a model similar to polycystic ovary syndrome

ClinicalTrials.gov ID NCT01690091)

These data indicate that genes associated with metabolic ROS generation tend to be upregulated in MZB compared to FoB, suggesting that differences in the control of ROS signaling might contribute to the classical distinctions between these two mature B cell subsets