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A construct of pcDNA3.1(+) GSTP1 containing a V5 tag at the NH 2 -terminus [pcDNA3.1(+) V5_GSTP1] was generated by PCR amplification of pcDNA3.1(+) GSTP1 using the forward primer: 5AAAAA GGATCC ACCATGGGTAAACCTATTCCTAATCCTCTTCTTGGTCTTGATTCTACA CCGCCGTATACCGTGG 3 with a BamHI restriction site indicated in italics, the V5 tag sequence is underlined and the GSTP1 sequence is in bold

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Prioritizing these questions is essential to move beyond descriptive phenomenology and towards the rational design of therapeutic strategies that can reliably steer this dynamic crosstalk toward tumor eradication