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The frequency of immune cell subsets was assessed by staining 1 x 10 6 nucleated cells with CD45 (KO), CD56 (PC7), CD138 (APC) (both Beckman Coulter), CD14 (PerCP), CD19 (APC-H7), CD3 (V450) and CD16 (PE) (all BD Biosciences)

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The diagnostic and prognostic value of tumor angiogenesis

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