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Description
These symptoms develop gradually because the body stores several years worth of B12 in the liver, meaning deficiency takes time to become clinically apparent
Immunofluorescence Staining and Quantification PBS-washed cells on coverslips were fixed with 4% paraformaldehyde (15 min, RT) and permeabilized with 0.1% Triton X-100 (5 min, RT)
Now I know from going through it that it couldnt be more true
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