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doi: 10.1345/aph.18252
The emerging role of nutraceuticals and phytochemicals in the prevention and treatment of Alzheimers disease

Cells were then washed twice with PBS and fixed in 4% PFA (Fisher Scientific 50-980-494) in PBS containing 0.1% Triton X-100 (PBST, Sigma-Aldrich T9284) for 20 min at room temperature at dark
The reason is that pairing it with Vitamin C yields significantly better results
