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Implementing prognostic and predictive biomarkers in CRC clinical trials

Science 186:11261128 Purpura DP (1975a) Dendritic differentiation in human cerebral cortex: normal and aberrant developmental patterns

Cells were cultured for 24 hours in the presence of CHIR99021, followed by 24 hours in either RPMI/B27 with or without insulin

It also allows for incredible flexibility in dosing