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Membranes were washed three times in TBS-T and incubated for 1 h with horseradish peroxidase-conjugated secondary antibodies: anti-rabbit IgG (1:5000, NA934, GE Healthcare, Bucks, UK) and anti-mouse IgG (1:5000, NA931V, GE Healthcare, Bucks, UK)

Its primary bioactive components include gastrodin (GTD), bisbenzyl compounds (e.g., 20c), and gastrodia polysaccharides (GEP) (Peng et al

2024) investigate the molecular mechanisms of CP-induced ototoxicity and the preventive effects of QU on it in male C57BL/6 J mice and PCs grown in vitro

Regardless of the dosage, your practitioner will likely suggest weekly shots for a few weeks
