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Approximate glutathione redox states can be estimated by simply comparing GS-bimane peak yields in aliquots from the same extract pre-treated or not with reductants like DTT, though the alkaline pH required for reaction with MBB probably causes some artefactual oxidation of extracted GSH if DTT is not present during the derivatization

That may include different treatments for a targeted approach
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There are many studies showing B-12 helps with depression and anxiety
