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For preparation of the membrane fraction the pellet was washed in 5 mL of 50 mM MES-KOH, 20 mM KCl, pH 6.5, centrifuged (150,000 g , 60 min at 4 C), resuspended in 500 L of the same buffer and finally sonicated (20%, 0.5 s 1 , on ice) until membranes were fully suspended
Notably, downregulation of the polycomb repressive complex (PRC2) histone methyltransferase gene EZH1 contributes to reduced gene silencing
However, AMPK can also promote ferroptosis by targeting BECN1 [50]

The MFSD12-T254A mutant inhibits MFSD12 function and suppresses tumor progression, whereas overexpression of MFSD12 worsens the prognosis of breast cancer patients
