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For the mixed spheroids, LEC were collected 48 h after transfection and labeled with cell membrane labeling lipophilic dyes CellVue Jade and CellVue NIR780 (88-0876-16 and 88-0875-16 respectively, Thermo Fisher Scientific)

These aspects are extensively detailed in a recent review[13]

L-Carnitine and its corresponding derivative containing 2 carbons in the acyl moiety (acetyl-L-carnitine) are taken up into cells via the organic cation transporter novel 2 (OCTN2) which cotransports L-carnitine with Na + [43, 44]

(2013) investigated various trace elements in eggs obtained from different housing or farming systems and eggs derived from organic farms had the highest concentrations of most elements