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The double mutant gcn4::KanMX4 met4::KanMX4 (Y1571) was obtained likewise using a diploid resulting from the crossing of two single mutants

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AMP-activated protein kinase mediates mitochondrial fission in response to energy stress

Together, these data indicate that FAO reprogramming in lung-associated macrophages occurs via a DUSP5-mediated mechanism in response to BCG infection and offers both DUSP5 modulation, and FAO inhibition, as potential therapeutic avenues to modify the severity of sequelae associated with Mtb infection