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The membranes were blocked in 5% not-fat milk or 5% BSA in TBS-T for 60 min at room temperature, incubated with the appropriate primary antibody (+ 4 C, overnight) and then with a secondary antibody conjugated with HRP (60 min at room temperature)

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All experimental procedures involving animals were approved by the Institutional Animal Care and Use Committee of Sun Yat-sen University