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Gel electrophoresis and transfer Cells were plated 24 h prior to harvest at 2.2 10 5 cells/mL in DMEM with 10% FBS and 0.2 g/mL puromycin in 10 cm plates

(2) Amplification of neuroinflammatory cascades: MPs/NPs activate the microglial NF-B pathway, inducing M1 polarization and pro-inflammatory cytokine release (TNF-, IL-1), ultimately resulting in dopaminergic neurodegeneration in the substantia nigra

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Gtarek P., SekulskaNalewajko J., BobrowskaKorczaka B., et al., Plasma Metabolic Disturbances in Parkinson's Disease Patients, Biomedicines 10, no