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Donkey anti-mouse alexa 594 secondary antibody (Invitrogen, UK) was applied for 1 h at 1:250 dilution, and auto-fluorescence was quenched using 0.01% Sudan Black B

Der Glutathionspiegel in den unteren Atemwegen von IPF-Patienten wurde mit dem von gesunden Teilnehmern verglichen, die allesamt Nichtraucher waren