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Before being embedded in paraffin wax, the muscle was fixed in buffered formalin (pH 7.4) for 24 h, decalcified with TBD-2 Decalcifier (ref
This timing requirement adds a layer of complexity to the protocol that newer compounds simply do not have
More recent peer-reviewed research has investigated AOD-9604 in chondroprotection and osteoarthritis models
10.1016/s0928-4257(97)89478-8 6 HuangT.ZhangK.SunL.XueX.ZhangC.ShuZ.et al (2015)
