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Finally, to determine whether Chac1 translated from the second methionine codon has catalytic activity, we transfected Chac1-Myc, Chac1-Myc and 5-Chac1-Myc into HEK293 cells

Finally, the authors looked at two other leukemia cell lines (Molt-4 and HL60) to investigate their effect on p53 expression
J.KrssakM.KautschitschS.SommergruberW.Tontsch-GruntU.et al (2008)