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Description
Protein in sample A and B was removed by precipitating with 200 L of cold ACN (containing 1 g/mL carbachol, the internal standard, IS) and centrifuging at 16,900 g for 20 min

A deficiency may lead to increased shedding, but excessive intake can also damage hair

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Norenberg MD, Rama Rao KV, Jayakumar AR (2009) Signaling factors in the mechanism of ammonia neurotoxicity
