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We use the Charles River Endosafe PTS system to test for bacterial endotoxins following USP guidelines: The following are suitability parameters that verify the system was working properly and the sample prep dilution is appropriate for accurate results
Water quality variables (dissolved oxygen, temperature, pH, total ammonia nitrogen, nitrite, alkalinity, and water use) will be monitored to assess system stability and biofiltration efficiency
Intratracheal instillation of active caspase-3 protein led to alveolar epithelial cell apoptosis and this disease development in mice (82)
