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The same results were obtained with two SQR knockout cell lines independently generated with different guide RNAs (Figure S3D)

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Then, cells were sedimented by centrifugation (14,000 rpm, 5 min) and resuspended in 1 mL of fresh 50 mM potassium phosphate buffer to measure the fluorescence using a Varioskanr TM LUX Multimode Microplate Reader (Thermo Fisher Scientific, Massachusetts, MA, United States)

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