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When the animals exhibit small movements confined to the forelimbs, without the involvement of the hindlimbs or pendulum-like swings due to momentum gained during earlier mobility phases [31], were recorded as Immobility

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The TNF- levels were determined using the rat TNF- ELISA kit from Boster Biological Technology (CA, United States), while IL-1 and IL-6 levels were measured using the rat IL-1 beta/IL-1F2 Quantikine ELISA Kit and the rat IL-6 Quantikine ELISA Kit from R&D Systems (MN, United States), respectively

Research shows that adding nutritional treatment to standard detox can increase success rates from 40% to 70%and in some cases, up to 81%
