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For sandwich ELISA, Maxisorp plates were coated overnight at 4 C with a polyclonal goat anti-HSA antibody (Sigma, # A-7544) diluted 1:1000 in PBS for the unfused HSAs or with monoclonal rabbit anti-camelid VHH antibody (Genscript, # A01860) diluted 1:1000 in PBS for the Albu-LiTE fusion proteins
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The results of B12 injections vary for each person based upon how much B12 they already receive daily
