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Available at: PubMed Sikiric P, Seiwerth S, Rucman R, et al
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The His6-SUMO tag was cleaved by ULP1 treatment, and the tag-free protein was fractioned with Ni-NTA column and Superdex 75size exclusion column (GE Healthcare) with 25 mM HEPES (pH 8.0), 300 mM NaCl, and 1% glycerol
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