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Sterile water works best when: The entire reconstituted peptide will be used in a single session Maximum purity without any additives is required for the protocol Cell culture experiments demand preservative-free conditions Budget constraints favor the more economical single-use option Sensitive assays might show interference from benzyl alcohol Research Application Considerations Cell culture applications present unique constraints on reconstitution solution selection

But that material is only as good as the hands it's in

Aono et al., 1993

Additional constraints include the potential for compounds with redox potentials higher than the Fe 3+ /Fe 2+ couple to overestimate values, and interference from sample chromophores absorbing at 593 nm (e.g., biliverdin), causing overestimation