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Under physiological conditions, ROS are generated in the heart mainly from the mitochondrial electron transport chain, nitric oxide synthases (NOS), NADPH oxidases (NOX), and xanthine oxidase (XO) in small quantities [7, 8]

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doi: 10.1093/infdis/jiad337 48 LvYChenDTianXXiaoJXuCDuLet al

The mice were anesthetized with 300 mg/kg chloral hydrate and were intratracheally injected with 3 mg/kg BLM (Meilun Biotechnology Co., Ltd., Dalian, China) in 100 l of saline via tracheostomy to induce pulmonary fibrosis
