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Caspase-Glo reagent was added to the wells according to the manufacturers instructions and luminescent signal was measured on a SpectraMax M3 microplate reader (Molecular Devices)

The specific joint or bursa treated, with its anatomical name and laterality (right knee joint or left subacromial bursa, never knee or shoulder alone)

A 12-week randomized trial demonstrated: Average weight loss of 2.6 kg in AOD-9604 group (1 mg/day) versus 0.8 kg in placebo group Preferential reduction in abdominal and visceral fat deposits No adverse effects on carbohydrate metabolism or insulin sensitivity Non-dose-dependent effects, with higher doses (10 mg/day) producing less weight loss than lower doses However, a subsequent 24-week Phase III trial with 502 subjects incorporating intensive diet and exercise showed no statistically significant weight loss compared to placebo, leading to development termination in 2007