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For instance, miR-22-3p and miR-149-5p bind the 3'UTR of MTHFR mRNA, and under folate deficiency, their upregulation suppresses MTHFR protein, further restricting one-carbon flux
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For viral infection assays, target cells were incubated with the virus for 1 h, after which the medium was replaced with fresh medium supplemented with 2% FBS until further experimentation
