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3.2.5 Effects on B lymphocytes HIIT acutely alters B-cell counts, leading to a transient decrease immediately post-exercise, followed by a return to baseline within 30 minutes (117)

The membrane was first blocked in 5% (wt./vol.) skim milk powder (Lab M Limited, Heywood, UK) in tris-buffered saline with 0.1% tween20 (TBST) for 1 h at RT, then was probed with the primary antibody (concentrations and manufacturers in Supplementary Table 4) diluted in TBST with 5% BSA for 16 h at 4 C on a rocker

( c ) MCF-7 cells cultured in 13 C-glucose for 5 days and switched to 12 C-glucose combined with 100 M kyn treatment for 6 h

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