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The bacteriostatic water provides some protection, but repeated access with imperfect sterile technique introduces cumulative contamination risk

Particularly, the cryo-EM structures of polymerases from filovirus and human metapneumovirus have no densities for the accessory regions in their L proteins even if the full-length L proteins are used for sample preparation and structural determination 8,9,32,33,34,35 , whereas the accessory regions attached to the core region can be observed in the structures of paramyxoviral and rhabdoviral polymerases 3,26,27,28,29,30,31