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Since overactivation of NMDARs is considered to be the main factor causing glutamate excitotoxicity ( In the pathogenesis of PD, regulation of the glutamate transporter gene is considered to be affected by epigenetic modifications

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The harvested cells were resuspended in buffer A (20 mM Tris-HCl, pH 8.0, 300 mM NaCl and 1 mM DTT) for GST-tagged proteins or in buffer B (20 mM Tris-HCl, pH 8.0, 300 mM NaCl, 5% glycerol and 10 mM imidazole) for His-tagged ShHTL7, and they were lysed by sonication

The significant increase in this important anti-oxidant is highly significant, because it is a major defense-line against oxidative damage which is common during lung infections