glutathione peroxidase 1 and heme Frontiers Interplay between Erythrocyte Peroxidases and

The diagnostic criteria for HE are: reduced autonomic activity, lethargy, delayed response, ataxia, coma and other symptoms in rats 57

MPO is a hemeiron-containing enzyme implicated in the pathology of chronic inflammation that, in the presence of hydrogen peroxide and chloride ions, will destroy its heme prosthetic group to generate free iron (Maitra et al

PBS and 100 g/mL CcSOD1 served as the blank and negative controls, respectively

Protein spots were excised and destained with 25 mM (NH 4 )HCO 3 /50% (v/v) acetonitrile (ACN), followed by an additional wash in 100% ACN using Xcise (Shimadzu), and then dried to enable ingel digestion