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What Is Schematic Design (SD)

doi: 10.1093/bja/aen078

Prior to imaging, seedlings were rinsed in sterile water and stained with 500 nM propidium iodide (PI, 50 M stock solution in H 2 O) for 1 minute, then rinsed again with water to get rid of the excess staining

Massage especially for the quadriceps muscle may also be helpful
