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Brain oxidative stress was determined as the GSSG/GSH ratio in hippocampus as previously described (Ezquer et al., Determination of Astrocyte and Microglia Immunoreactivity Following continuous chronic ethanol intake for 48 days, animals were alcohol deprived for 14 days and treated for the last 4 days of deprivation with either LY341495/NAC, SZ/NAC, LY341495/vehicle, SZ/vehicle, or vehicle/vehicle and then offered ethanol access for two consecutive days

When the concentration of glutathione in milk and ewe's milk was 0.02 mg/mL, the IR characteristic absorption peaks at 1,654 and 1,533 cm 1 were still visible, which were generated by the carbonyl C=O bond stretching (amide I) and CNH bond bending (secondary amide II) vibrations, respectively

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These properties position RMT as a suitable approach for therapeutics requiring precise targeting and sustained brain exposure