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Cathepsin cysteine proteases are effectors of invasive growth and angiogenesis during multistage tumorigenesis
Briefly, metabolites from 3 10 6 cells were extracted on ice with 0.8 ml pre-cold 80% methanol (80 C), and cell rupture was achieved by a Bullet Blender Storm 24 (Next Advance, Troy, NY) with metal beads, followed by five freeze-thaw out cycles
In contrast, POMGnT1, another GlcNAc transferase acting on O -mannose, has both an N-terminal lectin domain and a C-terminal GT-A fold 40
