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The cells were transfected with 3 g of AKT-siRNA (RIBOBIO, China) in 240 L OPTI-MEM Reduced Serum Medium for 57 h using a previously reported procedure
Nonetheless, because autism is influenced by multiple interacting genetic and environmental factors that are case-specific and inherent limitations in post-mortem brain, these observations will require confirmation in subsequent studies

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Zhang, C., Liu, J., Wang, J., Hu, W
