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However, GCLC alone does have enzymatic activity as Gclm knockout mice are viable but have markedly reduced tissue GSH levels (reduced by about 85 to 90%) [44]

The safest protocol is dosing 12 hours before drinking begins and again after drinking stops, allowing hepatic stores to be maximised when acetaldehyde accumulation is highest

Evidence: This distinction is consistent with the well-established, zero-order kinetics of alcohol elimination the liver breaks down alcohol at a roughly fixed rate (commonly cited around 0.015% BAC per hour) regardless of how much water, coffee, or food is consumed afterward