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Samples were diluted with four times the initial sample volume with 50 mM ammonium bicarbonate, and 1 l of a 1% w/v solution of Protease Max Surfactant Trypsin Enhancer was added [39], followed by digestion with sequencing grade-modified trypsin at a ratio of 1:25 (protease:protein) overnight at 37 C

This allergic reaction is a medical emergency

This results in somewhat empirical material design, making precise regulation difficult to meet the different requirements for dominant storage mechanisms in specific application scenarios

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