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After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions

It was also shown to attenuate fatigue in endurance athletes in a small placebo-controlled trial

They are yellow, orange, and red organic compounds composed of eight isoprene units and characterized by many conjugated double bonds

Plates were incubated at 30 C for 24 days to estimate colony-forming units and for spot assays