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These steps are intended to stabilize intermediate folding or unfolded states of the proteins, which may in turn facilitate the refolding reactions to be more productive 21

Practical Considerations Titration may be needed: Starting lower and building up could minimize GI side effects

Notwithstanding the likely differences between the substrate specificity between different species, our findings support the consideration of minipig systems as a complementary assay to be used in conjunction with the traditional Ames test

Common sites: lower abdomen (2 inches from the navel), upper thigh, or proximal to the target injury