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In mammalian ageing models, particularly D-galactose-induced mice, polysaccharide supplementation consistently increased SOD, CAT, and glutathione peroxidase (GPx) activities by 2045%, reduced malondialdehyde (MDA) by 2540%, and improved learning ability and physical performance [32,132,133]

After incubation for 10 min, 200 L luciferin solution (containing 20 mM tricine, 1.0 mM MgSO4, 0.1 mM EDTA, 33 mM dithiothreitol, 270 M coenzyme A, 500 M D-luciferin, and 530 M ATP, pH 7.8) was added, and then the firefly luminescence was measured with a BioTek synergy 4 Multi-Mode Microplate Reader

Large deformed lumps of fat and fibrotic tissue/swelling have developed asymmetrically on the legs which can impede normal movement of the limb