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Strains for LpoB complementation titers and growth curve assays were prepared as above, through CRIM-integration of LpoB variants under the control of the IPTG-inducible lac promoter (Plac) into the background of a lpoB deletion and ponA depletion strain (CB4) 30 and selection with 10 g/mL tetracycline (tet 10 ) on LB-agar supplemented with 0.2% arabinose

[DOI] [PubMed] [Google Scholar] 50.Czeredys M, Samluk L, Michalec K, Tulodziecka K, Skowronek K, Nalecz KA

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Polygon selection tool was used to select the area to be quantified and average pixel intensity was calculated with the analyze-measure command