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Mottaran E, Stewart SF, Rolla R, Vay D, Cipriani V, Moretti M et al

Overcoming barriers in the study of tight junction functions: from occludin to claudin

The following primary antibodies were added directly to the blocked samples and incubated for 30 min at 4 C: CD45-PerCP (BD Biosciences, Cat# 557235 1:100), CD11b-FITC (BD Biosciences, Cat# 553310 1:100), F4/80-PE (BD Biosciences, Cat# 563899 1:50), Ly6C-APC (BioLegend, Cat# 128003 1:25) and MHCII-PE-Cy7 (BioLegend, Cat# 116419 1:100)

Figure 10 In addition to the sulfonamides, e.g., 40 and 41 , the spiro-oxindole dihydroquinazolinone derivative 42 was identified in the screening campaign and subsequently a large series of related analogues were made, e.g., by applying rapid MW-assisted reactions and thereafter examined as IRAP inhibitors in bioassays (Engen et al., 2020) (Figure 10)