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After aspiration and washing, 100 l of substrate solution was added to each well and incubated for 30 min at room temperature. Then, 100 L of a stop solution was added, and color changes were measured at 450 nm using a BioTek ELISA reader

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In summary: Methylation is a biochemical process that is important in gene expression and repair

These findings suggest that chromium supplementation may be beneficial in managing insulin resistance in PCOS patients ( Besides metabolic outcomes, chromium supplementation has also been tied to beneficial reproductive outcomes in PCOS