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In recent years, advancing research has increasingly highlighted the pivotal role of TMAO in human health across multiple domains

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Invest Clin 2007;48(2):243-248

Brains were homogenized using lysis buffer for whole protein and mitochondrial protein extraction as previously described (Nguyen et al., Novex 4%12% Bis-Tris gels (Life Technologies, Carlsbad, CA, USA), then transferred to PVDF membranes (Rockford, IL, USA), which were blocked with non-fat milk and incubated with primary antibodies (OXPHOS complexes