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Samples were pooled together and centrifuged at 200 g for 5 min and filter sterilized via 0.2 m PES filters

Li X, Li M, Zhou B, Yang Y, Wei Q, Zhang J (2019) Transcriptome analysis provides insights into the stress response crosstalk in apple ( Malus domestica ) subjected to drought, cold and high salinity

Enzyme-linked immunosorbent assay (ELISA) For comparing antigenicity, purified StrepII-tagged E2 (1.0 g/mL in TBS) or E2E1-I53-50A (equimolar E2, i.e., 2.1 g/mL in TBS) were coated for 2 h at room temperature on 96-well Strep-TactinXT coated microplates (IBA LifeSciences)