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The nuclei were counterstained using DAPI (Thermo Fisher Scientific), after which the stained sections were imaged with a confocal microscope (LSM880, Zeiss)

154 Current estimates suggest that 95% of mammalian serotonin is localized within the gastrointestinal tract, and only 3% of dietary tryptophan is utilized for serotonin synthesis in the body

Upregulation of matrix metalloproteinase 9 (MMP9)/tissue inhibitor of metalloproteinase 1 (TIMP1) and MMP2/TIMP2 ratios may be involved in lipopolysaccharide-induced acute lung injury

It also supports mitochondrial stabilization and provides neuroprotective support with aging