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Eysiaks et al
Fluorescence intensity was read at excitation 550 nm, emission 600 nm for red fluorescence and at excitation 485 nm, emission 535 nm for green fluorescence using an M5E fluorescence plate reader
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Furthermore, immunofluorescence analysis of spermatogenic cell specific markers indicated that C18-4 and GC-1, markers of spermatogonial stem cells, decreased more than markers of other spermatogenic cells following busulfan treatment, indicating that type A and B spermatogonial stem cells were more vulnerable to busulfan (39)
